Pokémon

Many Pokémon made appearances in Ash Ketchum vs Charles Darwin. The Pokémon that appeared throughout the battle were hand-drawn animations.

Cameos in the group

 * Charizard
 * Butterfree
 * Alolan Exeggutor
 * Pikachu
 * Lapras
 * Magikarp

Trivia

 * As a group, they are the fifth video game characters to appear, after Mario, Luigi, Princess Peach, and Edward Kenway.
 * They are the third video game characters too be a cameo, after Princess Peach and Edward Kenway.
 * They are the first video game characters to be a group cameo.
 * All the Pokémon that appeared throughout the battle first debuted from the first generation of Pokémon, with the exception of Alolan Exeggutor, an alternate form of Exeggutor whom debuted in the first generation as well.
 * While not physically appearing, Jigglypuff was directly mentioned during the line "You're soft like a Jigglypuff", and Squirtle was referenced in the scrapped line "I've been catching critters before you were a Squirtle in your daddy's Poké Balls".
 * MissingNo., a glitch Pokémon noncanonical to the series, was also indirectly referenced during the line "You're a glitchy old man best left out at sea".